SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. Transcreener ® ADP. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. SpectraMax Paradigm Multi-Mode Safety Guide The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax M2. on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Figure 2 shows a Lck kinase dilution curve obtained using the FAM-p34cdc2-derived peptide. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 25 fmol/well 384-well plate) Filters/Mirrors: Fluorescein 1 pM typical (0. In the presence of ACE, the PL intensity was increasing by time, while the PL intensity sustained a low and stable fluorescence in 3 hours in the absence of ACE. 5%in96-and384-wellmicroplates. 0 out of 5 $ 9,995 $ 9,495. Other technologies such as. The microplate reader will read the plate and the relative fluorescence units will be displayed in the Plate section. $ 9,499. The percent The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant behaviour by TRAF6/NF-κB inhibition Pharmaceutical Biology December 31, 2022 Dang-ke. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SPECTRAmax® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics that permit experimental determinations at low-level detection limits. Plate reader settings for NanoOrange assay. SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Multimode Readers Comparison Chart. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a. ZERO BIAS - scores, article reviews, protocol conditions and moreFluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. NanoQuant plate. The instrument that I am using is Spectramax M2. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. 2. Read the microplate Step 1: Place the microplate in the reader. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. B. for the plate. SpectraMax® Microplate Readers and SoftMax® Pro Software. 1 SoftMax Pro v4. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Molecular Devices SpectraMax M2 Multilabel Microplate Reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Table 1. The plate reader. The SpectraTest fluorescence test plate (FL-1) can be used by Gemini XPS & M2 readers. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader , SpectraMax M5. Select the lab user profile. duAl monochromAtors With SpectraMax M2/M2e. On the SpectraMax iD5 reader, install the 320/100 excitation filter and 616/10 and 665/10 emission filters, and then select these filters from the Excitation and Emission dropdown wavelength menus. E Models. Self Service Available. 9000-0756. Add to Cart. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. spr SpectraMax iD5 SpectraTest ABS2 v1. 53 - 60 kg, (117-132 lb), depending on configuration. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. patent nos. item 1 Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O $4,999. Step 5: Click the software’s Read button. It is typically used to determine the concentration of nucleic acids and proteins. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. M2. 25 to 3. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 1) A maximal fluorescence intensity of 177 ± 3 RFU was observed at the highest cell concentration (Table 1). Appendix A. Align the two slots on the bottle holder with the two pegs on the rail. Microplate Readers. Dose-dependent enzyme essay Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. SoftMax Pro. A solution for low volume applications. 验证检测 SpectraMax 190 的工作状态。 堆板机、机械臂一体化整合 SpectraMax 190 可以在几分钟内整合到 Molecular Devices 的 StakMax 堆板机上。为了实现更高程度的自动化，自动化供应商 合作伙伴已经把我们的酶标仪系统和所有主要合作伙伴的机械臂 进行了整合。I am interested in using a SpectraMax M2 Plate Reader for a spectrophotometric assay and am trying to collect the proper reagents and materials. . By employing dyes that specifically bind to nucleic acids, DNA/RNA concentrations can also be quantified using fluorescence detection. Learn how you can utilize microplate readers to drive landmark discoveries. A. Auto-calibration and self-diagnostics. 1 New Reader Support 1. D 47 7. Temperature Control: ambient from +4 to 45C. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. Detection Ranges: absorbance 200-1000nm, fluorescence Ex: 250-850nm / fluorescence Em: 360-850nm, luminescence: 360–850 nm. FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. I understand the OD measurement in a plate reader varies with the volume of the sample measured. VANTAstar™ Flexible Multi-mode Microplate Reader. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 5008531B 3 Contents Chapter1:SpectraTestMulti-ModeValidationPlateOverview 4 Multi-ModeProtocols 6 Chapter2:GettingStarted 8 CertificateofCalibration 8SpectraMax M2. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Select the Driver tab and click Update Driver. The Spectramax i3 is a muti-mode plate reader used for measuring absorbance and fluorescence in microtiter plates. 0 out of 5 $ 9,995 $ 9,495. 2. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. A). g. The SpectraTest absorbance test plate (Abs-1) can be used by VersaMax, SpectraMax, & M2 readers. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. It does not work as expected. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. td. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. Listing: #4772697. 67. Figure 2. The plate reader optics are tunable for absorbance. 6 % of reading 4. Fluorescent Polarization: Solid black plates, as above. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR. as a fluorescence blank. . I've got a LT4500 microplate reader and was hoping to digest the pellets in papain overnight, simply mix a measure of that sample with DMMB solution in a 96 well plate and read absorbance at 525nm. Optimizing Time-Resolved Fluorescence Read Mode The SpectraMax M2 enables you to read Time-Resolved Fluorescence assays from the top of a plate. 0 out of 5. Absorbance (monochromator) Fluorescence (monochromator; FI, TR, FP) Luminescence; Molecular Devices Spectramax M2. Appendix. Description 1. 3) At the bottom of the strip, click on "Show. D 59 A. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Three data types for FP read types are also being introduced with v4. 0) OD (500 to 1100) RFU (5. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. For a higher . 7. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. These lab workhorses are used in drug discovery. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). After the plate is read, data will appear in the plate. (a) Chemical structures of 1 (HADP) and its analogues 2–4. Customer Satisfaction is our number one goal All lab equipment in stock already tested and ships in 1-3 days The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. 1. For SpectraMax L or LMax II, LMax II. Fig 2: Enzymatic response of synthetic compounds. Five preferred detection modes. Our absorbance plate readers feature our PathCheck Sensor technology and. 7. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The plate is set up for reading and images are acquired according to specified parameters. The first step consisted of taking an initial fluorescence measurement using Molecular Devices SpectraMax® M2 fluorescence plate reader of a 96-well plate containing PET-RAFT polymerization reaction mixtures in individual wells. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. com 10. A microplate reader as individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Plate reader set to 50 reads. 0. #4513: Plate Reader: A multi-detection microplate reader configured to measure the output of the UDP tracer is required. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Highly selective for dsDNA over ssDNA or RNA. Third this recommendation. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Add to Cart. Temperature variation ± 0. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and. (a) Chemical structures of 1 (HADP) and its analogues 2–4. Typical settings forTo maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Whether fluorescence, chemiluminescence, or visible detections - select the optimal plate for your assay!If your microplate reader requires an adapter for top reading (e. Detection modalities include absorbance (UV-Vis Abs) and. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). spr SpectraMax iD3 SpectraTest ABS2 v1. Basic "FL Plate Reader start up protocol" (print and bring along for the. 0 Concentration Dynamic Range 105 RFU MT oltage ignal Adjustable PMT gain High. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. The flexibility of a high end multimode plate reader with environmental control. Filters: Excitation and emission slides for 4 filters each. 7°C (gradient heating design). We suggest Corning 384-well plates Cat. U. Microplate Reader Shelf. olecular saka. Therefore, ADP production is proportional to an increase in fluorescence. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Plate Shaking. Fluorescence read mode and Endpoint read type are already selected in the pre-configured protocol. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. BMG Labtech - THERMOstar. To schedule a training session please contact Ewa Folta-Stogniew. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemFAST AND ACCURATE METERTECH Microplate Reader $3,650. The SpectraMax 190 reader can be integrated with Molecular . Fig 2: Enzymatic response of synthetic compounds. 3 foot) See. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. 8 Customer Reviews. 1. 7°C and the bottom to 36. Adjusting fluorescence gain on microplate readers. Multimode microplate readers offer flexibility, and ease of use. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. Multimode microplate readers deliver both performance and economy. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. FI assay. S. Analyze the data Step 1: After the microplate has been read, theFluorescence analysis was performed using a SpectraMax iD3 multi-mode plate reader (Molecular Devices LLC, U. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Brand: MOLECULAR DEVICES. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The entry should be SpectraMax GEMINI EM. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Fluorescence Microplate Readers. Microplate Formats: 6, 12, 24, 48, 96, 384. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. Then the working solution (50μL) was added to the plate for 30 min treatment without light at room temperature and then the stop solution (50μL) was added. WinKQCL ® Endotoxin Detection and Analysis Software Version 6 Features. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideReader Documentation Version No. com Setup for Omnia® assays on SpectraMax®. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. After washing with PBS, the fluorescence intensities for each staining were analyzed with a fluorescence plate reader (SpectraMax M2). Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. 9000-0756. The chamber. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190. spr SpectraMax Plus 384 SpectraTest ABS2 v1. Fluorescence measurements no longer require you to adjust the gain. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard plate reader applications, protecting your initial investment. Analyze the dataHere we report the use of the EarlyTox ™ Live/Dead Assay Kit in combination with SpectraMax ® fluorescence microplate readers (Figure 1). Reading Capabilities: cuvette or microplate. was 1. • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. The fluorometric. Email. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. refurbished. . Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. 6% for the on-board optics (monochromators). Endpoint, kinetic, spectrum and area-well. Description 1. CO 2 & O 2 control. 5°C at 37°C. Type Clear: Add to Cart. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. The instrument will read the plate, then the relative fluorescence units (RFUs) will be displayed in the Plate section of SoftMax Pro Software. WinKQCL ® Software can be used with a compatible microplate reader to run the template and. Documentation:Plate readers that are used to detect luminescence, fluorescence and absorbance signals can vary in sensitivity (Figure 2). For paper-based measurements, we used the Milenia HybriDetect 1 (TwistDx) lateral flow system, as per the manufacturer's. The software is easy to use and contains settings for hundreds of commonly used assays, such as BCA and MTT. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. We would like to show you a description here but the site won’t allow us. The method is based on. S. The assay is carried out in a standard 96-well plate. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for. 100 readings per well gives optimal Z’ values for this assay. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. Temperature control range from Ambient +4º to 50ºC. Real-time experimental control (REC) that automatically activates a procedure as soon as a specific data value is reached. Spectramax Multi-Mode Microplate Reader Performance Specifications. BioTek’s Cytation™3, for instance, is a multimode plate reader that also functions as a high-speed automated digital fluorescence microscope with brightfield capability. D 3 1. When displaced, changes in fluorescence can be read in an FP, FI, or TR-FRET depending on the assay format. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Molecular devices SpectraMax M5 Pdf User Manuals. Bioz Stars score: 86/100, based on 1 PubMed citations. 00. SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. Parameter EarlyTox Live/Dead EarlyTox Glutathione EarlyTox Caspase-3/7 R110 Read mode Fluorescence Fluorescence Fluorescence Read type Endpoint Endpoint Endpoint Wavelengths Lm1:. S. SpectraMax ® M2 Multi-Mode Microplate Reader. 0) OD (0. Multimode Microplate Readers. STAKMAX. . MOLECULAR DEVICES SPECTRA MAX GEMINI XS MICRO. 2018. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 Instrument settings used with the SpectraMax M5 Microplate Reader. DNA quantification with fluorescence dyes. We use Spectramax M5 microplate reader in our lab. USING OLIGREEN REAGENT IN GEMINI XS, GEMINI EM AND SPECTRAMAX M2 MICROPLATE READERS Step 6: Add 100 µL of each concentration of oligonucleotide to the wells of the microplate in triplicate. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize. This opens the Settings window. Luminex Magpix. SoftMax Pro Software has been integrated by many leading robotics and LIMS partners, enabling both data analysis and. If you are going to read it from the bottom, you should use a clear-bottom black plate. 5). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Python 3. Appendix. A microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. It can measure absorbance from 230 -1000 nm. g. The dual monochromator optics allow the. Microplate Readers. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. In addition, the SpectraMax iD5Popular answers (1) If you are going to read the plate from the top, you should use a solid black plate. The Cut-off filter for this pair is showing 530nm in this machine. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. Fluorescence is measured at time zero and again after one-hour of incubation at 37°C in a fluorescence microplate reader. The SpectraMax i3x reader provides theThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the Alexa594 tracer. Microplate Capacity. A whole well fluorescence imaging system that can collect information from all cells inside the well, from 6- to 384-well formats. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. This can increase throughput and efficiency, thus saving time and space in the lab. Figure 6: Transcreener ADP2 FI assay principle. SpectraTest FL1 Fluorescence Validation . 100 readings per well gives optimal Z’ values for this assay. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Absorbance; Fluorescence (FI, TR, FP) Luminescence. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. g. Note: Check the Applies to section at the bottom of the page to make this is the proper driver for your plate reader. Absorbance (monochromator) Fluorescence (monochromator; FI). D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Luminescence, and Fluorescence microplate re aders—providing extensive data calculation. Other technologies such as imaging. These measurements can include parameters such as absorbance, fluorescence intensity and. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. Avoid losing high signal. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Also refer to this Multimode Reader Comparison Chart. Plate Shaking Linear, orbital, double-orbital Dimensions (H x W x D) 12 x 15 x 15 in. An assay plate containing a 15-point standard curve was read on the SpectraMax. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® Assays. 25 to 3. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 8 is required to operate the new SpectraMax® M5 reader and its five read types: Fluorescence Intensity, Absorbance, Luminescence, Time-Resolved Fluorescence (TRF) and Fluorescence Polarization. Dual monochromators allow selection of any absorbance wavelength between 200 nm and 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. An approach to gain setting that I like to use is to set the gain based on the sample with the most fluorescence in order to get about 30-50% of the maximal signal before the detector saturates. The drawing shows a standard microplate of 15 mm height covered with a lid. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. luminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. A solution for low volume applications. 04 to 3. Add to Cart. , a positive control). SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). the reader’s stated range. The SpectraMax iD3 reader detects fluorescence using monochromators only. Next add 100 µl of workingThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time. 0 × 107) RLU 0. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Optimized Settings to Validate the Molecular Devices SpectraMax® M2/M2e Microplate Readers with the Transcreener® Fluorescence Intensity Assays. Model: SpectraMax M2. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch. 47 and 0. Figure 2. The following general steps are taken with our absorbance and fluorescence readers. Enables accurate fluorescence data with every run. 25 to 3. Absorbance cuvette port. the reader’s stated range. Description 1. Figure 6: Transcreener ADP2 FI assay principle. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. 1) Select the correct plate and the wells that you want to measure. 0) OD (0. Step 2: Click SoftMax Pro’s Read button. Prior to fluorescence analysis, the lid from the OmniTray plate was removed. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. L evices evices orporation. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. ~ Bio-Tek ELx808 Microplate Reader $2,795. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The Infinite® M1000 from TECAN is a high-end multimode Microplate Reader with premium Quad4 Monochromators. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. The plate reader offers a fast, simple readout of average fluorescence per well, while the cytometer gives more detailed information, including total cell number and.